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Plant Physiology 78:673-677 (1985)
© 1985 American Society of Plant Biologists

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Metabolic Conversion of Amino Acids Loaded in the Vacuole of Chara australis Internodal Cells 1

Katsuhiro Sakano and Masashi Tazawa

Department of Botany, Faculty of Science, University of Tokyo, Hong, Tokyo 113, Japan

Vacuoles of internodal cells of Chara australis (or Chara corallina) were loaded with a 10 millimolar amount of various amino acids by a perfusion method and incubated under continuous light. After 20 to 24 hours, the cell sap was collected, and free amino acids in it and the rest of the cell (cytoplasm) were analyzed. The only amino acid metabolized completely was alanine. About 40 to 80% of the aspartic acid, glutamine, serine, and glycine were metabolized, whereas less than 30% of the threonine, asparagine, isoasparagine, isoleucine, phenylalanine, {gamma}-aminobutyric acid, lysine, and arginine were metabolized. The figure for glutamic acid fluctuated between 10 and 100%. The main metabolites of alanine were glutamine, glycine and ammonia, which accumulated in the vacuole. Alanine utilization was not affected by L-methionine-D,L-sulfoximine or azaserine, but was strongly inhibited by aminooxyacetate. The cell extract contained enough alanine aminotransferase activity to account for the rate of alanine metabolism.

1 Supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture of Japan (No. 58340036), and by Special Coordination Funds for Promoting Science and Technology from the Science and Technology Agency of Japan.




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T. Gebbing and H. Schnyder
Pre-Anthesis Reserve Utilization for Protein and Carbohydrate Synthesis in Grains of Wheat
Plant Physiology, November 1, 1999; 121(3): 871 - 878.
[Abstract] [Full Text]


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Copyright © 1985 by the American Society of Plant Biologists