Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 79:80-83 (1985)
© 1985 American Society of Plant Biologists

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Articles

Polyamines and Root Formation in Mung Bean Hypocotyl Cuttings 1

II. Incorporation of Precursors into Polyamines

Ra'Anan Friedman2, Arie Altman and Uriel Bachrach

Department of Horticulture, The Hebrew University of Jerusalem, Rehovot 76100, Israel, Department of Molecular Biology, The Hebrew University and Hadassah Medical School, Jerusalem, Israel

The incorporation of [14C]arginine and [14C]ornithine into various polyamines was studied in mung bean (Vigna radiata [L.] Wilczek) hypocotyl cuttings with respect to the effect of indole-3-butyric acid on adventitious root formation.

Both [14C]arginine and [14C]ornithine are rapidly incorporated into putrescine, spermidine, and spermine, with similar kinetics, during 5- to 24-hour incubation periods. The incorporation of arginine into putrescine is generally higher than that of ornithine. The biosynthesis of putrescine and spermidine from the precursors, in the hypocotyls, is closely related to the pattern of root formation: a first peak at 0 to 24 hours corresponding to the period of root primordia development, and a second peak of putrescine biosynthesis at 48 to 72 hours corresponding to root growth and elongation. Indole-3-butyric acid considerably enhances putrescine biosynthesis in both phases, resulting in an increase of the putrescine/spermidine ratio.

It is concluded that the promotive effect of indole-3-butyric acid on putrescine biosynthesis, from both arginine and ornithine, supports the hypothesis that auxin-induced root formation may require the promotion of polyamine biosynthesis.

2 This work was submitted in partial fulfillment of the requirements for Ph.D. degree (R. F.), The Hebrew University.

1 Supported by a grant from the United States-Israel (Binational) Agricultural Research and Development Fund (BARD).






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