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Plant Physiology 79:365-370 (1985)
© 1985 American Society of Plant Biologists

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Articles

Effect of Triacontanol on Chlamydomonas1

II. Specific Activity of Ribulose-Bisphosphate Carboxylase/Oxygenase, Ribulose-Bisphosphate Concentration, and Characteristics of Photorespiration

Robert L. Houtz2, Stanley K. Ries and N. E. Tolbert

Department of Horticulture, Michigan State University, East Lansing, Michigan 48824, Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824

Increased photosynthetic CO2 assimilation by Chlamydomonas reinhardtii cells treated with triacontanol (TRIA) was not due to changes in glycolate excretion, CO2 compensation point, or the sensitivity of photosynthetic CO2 assimilation to O2. Kinetic analysis of TRIA-treated cells showed that the increase in photosynthetic CO2 assimilation was a result of an increase in the apparent Vmax for intact cells. The total activity of ribulose-P2 carboxylase/oxygenase was higher in cell lysates from TRIA-treated cells. However quantification of this enzyme concentration by binding of [14C]carboxyarabinitol-P2 did not show an increase in TRIA-treated cells. Thus, there was an increase in the specific activity of ribulose-P2 carboxylase/oxygenase extracted from Chlamydomonas cells treated with TRIA. TRIA alone had no effect on the activity of the enzyme in cell lysates from Chlamydomonas or purified from spinach (Spinacia oleracea L.) leaves.

The ribulose-P2 pool was 50 to 60% higher in cells treated with TRIA that were assayed for photosynthetic CO2 assimilation at high- and low-CO2. TRIA also increased ribulose-P2 levels in the absence of CO2 in the light with atmospheres of N2 or N2 with 21% O2.

2 Present address: Department of Horticulture and Landscape Architecture, University of Kentucky, Lexington, KY 40546.

1 Supported in part by National Science Foundation grant PCM 8005917 and published as Michigan Agricultural Experiment Station Journal Article 11534. This research is part of a Ph.D. thesis by R.L.H.






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Copyright © 1985 by the American Society of Plant Biologists