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Plant Physiology 79:436-440 (1985)
© 1985 American Society of Plant Biologists

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Articles

Pyruvate Decarboxylase from Zea mays L. 1

2. Examination of Hysteretic Kinetics

Pat J. Langston-Unkefer2 and Thomas C. Lee3

Department of Agronomy, University of Wisconsin, Madison Wisconsin 53706, United States Department of Agriculture, ARS, Madison Wisconsin 53706, Los Alamos National Laboratory, INC Division, Los Alamos, New Mexico 87545

A significant lag phase was observed in the accumulation of product for the reaction catalyzed by pyruvate decarboxylase (PDC) purified from mature maize kernels. The effects of pH, pyruvate, potassium chloride, PDC concentration, and Mg2+-thiamine pyrophosphate upon this lag and upon the observed cooperativity were investigated. PDC preincubated with Mg2+-thiamine pyrophosphate for six days had Michaelis-Menten kinetics, a Hill number of 1, and no apparent lag phase. The degree of saturation of PDC with Mg2+-thiamine pyrophosphate appears to have a central role in controlling the lag phase and the degree of cooperativity.

2 Present address: INC-4, MS C345, Los Alamos National Laboratory, Los Alamos, NM 87545.

3 The investigations reported were included in the dissertation submitted by T. C. L. to the Graduate College, University of Wisconsin, Madison in partial fulfillment of the requirements for the Ph.D. degree.

1 Supported by United States Department of Agriculture, Competitive Research Grants-CRCR-1-1201, the University of Wisconsin, and United States Department of Energy.






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Copyright © 1985 by the American Society of Plant Biologists