Plant Physiology 79:458-467 (1985)
© 1985 American Society of Plant Biologists
Articles
Enzymic Capacities of Purified Cauliflower Bud Plastids for Lipid Synthesis and Carbohydrate Metabolism 1
Etienne-Pascal Journet and
Roland Douce
Physiologie Cellulaire Végétale, DRF, Centre d'Etudes Nucléaires et Université Scientifique et Médicale, 85X F38041 GRENOBLE Cedex FRANCE
Isolated cauliflower (Brassica oleracea) bud plastids, purified by isopycnic centrifugation in density gradients of Percoll, were found to be highly intact, to be practically devoid of extraplastidial contaminations, and to retain all the enzymes involved in fatty acid, phosphatidic acid, and monogalactosyldiacylglycerol synthesis. Purified plastids possess all the enzymes needed to convert triose phosphate to starch and vice versa, and are capable of conversion of glycerate 3-phosphate to pyruvate for fatty acid synthesis. They are also capable of oxidation of hexose phosphate and conversion to triose phosphate via the oxidative pentosephosphate pathway. Cauliflower bud plastids prove to be, therefore, biochemically very flexible organelles.
1 Supported in part by Research Grant from the Centre National de la Recherche Scientifique (U.A. 576: Interactions Plastes-Cytoplasme-Mitochondries).
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