Plant Physiol. Illumina
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Plant Physiology 79:579-583 (1985)
© 1985 American Society of Plant Biologists

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Regeneration in Alfalfa Tissue Culture

Stimulation of Somatic Embryo Production by Amino Acids and N-15 NMR Determination of Nitrogen Utilization

Thomas A. Skokut, Jill Manchester and Jacob Schaefer

Monsanto Agricultural Products Company, St. Louis, Missouri 63198, Physical Sciences Center, Monsanto Company, St. Louis, Missouri 63167

The production of somatic embryos in alfalfa (Medicago sativa L., cv Regen S) is increased 5- to 10-fold by alanine and proline. However, utilization of nitrogen for synthesis of protein from alanine, proline, glutamate, and glycine is not qualitatively different, even though the latter two amino acids do not increase somatic embryo formation. These determinations were made by 15N labeling with detection by nuclear magnetic resonance. Overall metabolism of the nitrogen of proline, alanine, glutamate, and glycine is also similar in two regenerating and nonregenerating genotypes with similar germplasm, except that the levels of free amino acids are consistently higher in the nonregenerating line. In addition, when regeneration is suppressed in either of the two regenerating lines, the level of intracellular free amino acids increases. This increased level of metabolites is the only direct evidence provided by analysis of nitrogen metabolism of differences between the regenerating and nonregenerating states in alfalfa.




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L. Cegelski and J. Schaefer
Glycine Metabolism in Intact Leaves by in Vivo 13C and 15N Labeling
J. Biol. Chem., November 25, 2005; 280(47): 39238 - 39245.
[Abstract] [Full Text] [PDF]


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Copyright © 1985 by the American Society of Plant Biologists