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Regeneration in Alfalfa Tissue Culture

Characterization of Intracellular pH During Somatic Embryo Production by Solid-State P-31 NMR

Physical Sciences Center, Monsanto Company, St. Louis, Missouri 63167

The composition of phosphorus-containing compounds of intact lyophilized alfalfa tissue has been determined, in part, by solid-state ³¹P nuclear magnetic resonance. The tissue (Medicago sativa L., cv Regen S; and some of its crosses) was grown in culture under both nonregenerating and regenerating conditions, the latter enhanced by the addition of specific amino acids. Analysis of the ³¹P nuclear magnetic resonance spectra shows that regeneration is favored when metabolism occurs without the production of a low average intracellular pH.

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