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Plant Physiology 79:667-671 (1985)
© 1985 American Society of Plant Biologists

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Identification of 3-(O-{beta}-Glucosyl)-2-Indolone-3-Acetylaspartic Acid as a New Indole-3-Acetic Acid Metabolite in Vicia Seedlings

Seiji Tsurumi1 and Shunji Wada

Biological Institute, Faculty of Science, Tohoku University, Sendai 980 Japan

A new indole-3-acetic acid metabolite was isolated from broad bean (Vicia faba L. cv Chukyo) seedlings. It was a conjugate of dioxindole-3-acetic acid, aspartic acid, and glucose and was identified as 3-(O-{beta}-glucosyl)-2-indolone-3-acetylaspartic acid (molecular weight 484) from ultraviolet, infrared, nuclear magnetic resonance, and mass spectra. Its natural content in 4-day-old Vicia seedlings was estimated to be 8.6 nanomoles per gram fresh weight. It was suggested that oxidation of indole-3-acetic acid not accompanied by decarboxylation might regulate endogenous level of the hormone.


1 Present address: Division of Science of Biological Resources, The Graduate School of Science and Technology, Kobe University, Rokkodai, Kobe 657, Japan.







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Copyright © 1985 by the American Society of Plant Biologists