This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (68) Citing Articles via Google Scholar Google Scholar Articles by Hrubec, T. C. Articles by Donaldson, R. P. Search for Related Content PubMed PubMed Citation Articles by Hrubec, T. C. Articles by Donaldson, R. P. Agricola Articles by Hrubec, T. C. Articles by Donaldson, R. P.

Articles

Effects of CO₂ Enrichment and Carbohydrate Content on the Dark Respiration of Soybeans ¹

Plant Photobiology Laboratory, Plant Physiology Institute, United States Department of Agriculture/Agricultural Research Service, Beltsville Agricultural Research Center-West, Beltsville, Maryland 20705, The Department of Biological Sciences, The George Washington University, Washington DC 20052

During the period of most active leaf expansion, the foliar dark respiration rate of soybeans (Glycine max cv Williams), grown for 2 weeks in 1000 microliters CO₂ per liter air, was 1.45 milligrams CO₂ evolved per hour leaf density thickness, and this was twice the rate displayed by leaves of control plants (350 microliters CO₂ per liter air). There was a higher foliar nonstructural carbohydrate level (e.g. sucrose and starch) in the CO₂ enriched compared with CO₂ normal plants. For example, leaves of enriched plants displayed levels of nonstructural carbohydrate equivalent to 174 milligrams glucose per gram dry weight compared to the 84 milligrams glucose per gram dry weight found in control plant leaves. As the leaves of CO₂ enriched plants approached full expansion, both the foliar respiration rate and carbohydrate content of the CO₂ enriched leaves decreased until they were equivalent with those same parameters in the leaves of control plants. A strong positive correlation between respiration rate and carbohydrate content was seen in high CO₂ adapted plants, but not in the control plants.

Mitochondria, isolated simultaneously from the leaves of CO₂ enriched and control plants, showed no difference in NADH or malate-glutamate dependent O₂ uptake, and there were no observed differences in the specific activities of NAD⁺ linked isocitrate dehydrogenase and cytochrome c oxidase. Since the mitochondrial O₂ uptake and total enzyme activities were not greater in young enriched leaves, the increase in leaf respiration rate was not caused by metabolic adaptations in the leaf mitochondria as a response to long term CO₂ enrichment. It was concluded, that the higher respiration rate in the enriched plant's foliage was attributable, in part, to a higher carbohydrate status.

This article has been cited by other articles:

				S. Legros, I. Mialet-Serra, J.-P. Caliman, F. A. Siregar, A. Clement-Vidal, D. Fabre, and M. Dingkuhn Phenology, growth and physiological adjustments of oil palm (Elaeis guineensis) to sink limitation induced by fruit pruning Ann. Bot., November 1, 2009; 104(6): 1183 - 1194. [Abstract] [Full Text] [PDF]

				S. Legros, I. Mialet-Serra, A. Clement-Vidal, J.-P. Caliman, F.A. Siregar, D. Fabre, and M. Dingkuhn Role of transitory carbon reserves during adjustment to climate variability and source-sink imbalances in oil palm (Elaeis guineensis) Tree Physiol, October 1, 2009; 29(10): 1199 - 1211. [Abstract] [Full Text] [PDF]

				N. Gomez-Casanovas, E. Blanc-Betes, M. A. Gonzalez-Meler, and J. Azcon-Bieto Changes in Respiratory Mitochondrial Machinery and Cytochrome and Alternative Pathway Activities in Response to Energy Demand Underlie the Acclimation of Respiration to Elevated CO2 in the Invasive Opuntia ficus-indica Plant Physiology, September 1, 2007; 145(1): 49 - 61. [Abstract] [Full Text] [PDF]

				H. Usuda Effects of Elevated CO2 on the Capacity for Photosynthesis of a Single Leaf and a Whole Plant, and on Growth in a Radish Plant Cell Physiol., February 1, 2006; 47(2): 262 - 269. [Abstract] [Full Text] [PDF]

				H. Sasaki, T. Hara, S. Ito, S. Miura, Md. M. Hoque, M. Lieffering, H.-Y. Kim, M. Okada, and K. Kobayashi Seasonal Changes in Canopy Photosynthesis and Respiration, and Partitioning of Photosynthate, in Rice (Oryza sativa L.) Grown Under Free-Air CO2 Enrichment Plant Cell Physiol., October 1, 2005; 46(10): 1704 - 1712. [Abstract] [Full Text] [PDF]

				J. A. BUNCE Response of Respiration of Soybean Leaves Grown at Ambient and Elevated Carbon Dioxide Concentrations to Day-to-day Variation in Light and Temperature under Field Conditions Ann. Bot., May 1, 2005; 95(6): 1059 - 1066. [Abstract] [Full Text] [PDF]

				M. A. GONZALEZ-MELER, L. TANEVA, and R. J. TRUEMAN Plant Respiration and Elevated Atmospheric CO2 Concentration: Cellular Responses and Global Significance Ann. Bot., November 1, 2004; 94(5): 647 - 656. [Abstract] [Full Text] [PDF]