Plant Physiol. Drug Metab Dispos
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Plant Physiology 79:760-764 (1985)
© 1985 American Society of Plant Biologists

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Articles

Selection and Characterization of Chlorella Mutants Deficient in Amino Acid Transport 1

Further Evidence for Three Independent Systems

Norbert Sauer2 and Widmar Tanner

Institut für Botanik, Universität Regensburg, Universitätsstra{beta}e 31, 8400 Regensburg, Federal Republic of Germany

Six amino acids are transported at high rates across the plasmalemma of Chlorella vulgaris only after the induction of two specific transport systems. Induction is achieved either by pretreatment with glucose, glucose analogs, or by nitrogen starvation. Mutants for these transport systems were obtained after incubation of Chlorella cells in the presence of acridine orange or ethidium bromide, followed by a selection procedure using the toxic amino acid analogs L-canavanine (for L-arginine), and L-azetidine-2-carboxylic acid (for L-proline). Mutants isolated by this method had lost their ability to induce the corresponding transport system. Double mutants deficient in transport of both these amino acids still possess the general amino acid transport system, a third system which was described previously. Evidence for additional amino acid transport systems in Chlorella is discussed.

2 Present address: Plant Biology Laboratory, The Salk Institute for Biological Studies, San Diego, CA 92138.

1 Supported by the Deutsche Forschungsgemeinschaft (SFB 43).






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Copyright © 1985 by the American Society of Plant Biologists