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Plant Physiology 79:1006-1010 (1985)
© 1985 American Society of Plant Biologists

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Articles

Nitrate Reductase of Green Algae Is Located in the Pyrenoid 1

Antonio Lopez-Ruiz, Jean Pierre Verbelen, Jose Manuel Roldan and Jesus Diez

Departamento de Bioquímica, Facultad de Veterinaria, Universidad de Córdoba, Córdoba 14071, Spain, Departamento de Bioquímica, Facultad de Ciencias, Universidad de Córdoba, Córdoba 14071, Spain, Departement Biologie, Universitaire Instelling Antwerpen, Universiteisplein 1, B-2610 Wilrijk, Belgium

Antibodies against nitrate reductase from Monoraphidium braunii have been used to determine the antigenic relationships of nitrate reductases from different green algae. Nitrate reductases from Chlamydomonas reinhardii, Chlorella fusca, Dunaliella salina, and Scenedesmus obliquus, were inhibited by, and cross-reacted with, antibodies raised against the enzyme from Monoraphidium braunii.

These antibodies were also used to determine, by immunoelectron microscopy, the intracellular location of nitrate reductase in the aforementioned green algae. In all cases, the enzyme was specifically located in the pyrenoid.

1 Supported by Comisión Asesora de Investigación Científica y Técnica Grant No. 1370/82, a grant of National Fund for Scientific Research (NFWO-Belgium) to J.-P. Verbelen, and an EMBO short-term fellowship to A. López-Ruiz.






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Copyright © 1985 by the American Society of Plant Biologists