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Plant Physiology 79:1080-1085 (1985)
© 1985 American Society of Plant Biologists

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Articles

Auxin Regulation of a Proton Translocating ATPase in Pea Root Plasma Membrane Vesicles 1

Reinhard Gabathuler2 and Robert E. Cleland

Department of Botany, University of Washington, Seattle, Washington 98195

Pea root microsomal vesicles have been fractionated on a Dextran step gradient to give three fractions, each of which carries out ATP-dependent proton accumulation as measured by fluorescence quenching of quinacrine. The fraction at the 4/6% Dextran interface is enriched in plasma membrane, as determined by UDPG sterol glucosyltransferase and vanadate-inhibited ATPase. The vanadate-sensitive phosphohydrolase is not specific for ATP, has a Km of about 0.23 millimolar for MgATP, is only slightly affected by K+ or Cl– and is insensitive to auxin. Proton transport, on the other hand, is more specific for ATP, enhanced by anions (NO3– > Cl–) and has a Km of about 0.7 millimolar. Auxins decrease the Km to about 0.35 millimolar, with no significant effect on the Vmax, while antiauxins or weak acids have no such effect. It appears that auxin has the ability to alter the efficiency of the ATP-driven proton transport.

2 Present address: Swiss Institute for Experimental Cancer Research, Department of Genetics, CH-1066 Epalinges s/Lausanne, Switzerland.

1 Supported by contract DE-AM06-76ER73019 from the United States Department of Energy and grant 80-CRCR-100407 from the United States Department of Agriculture.




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Y.-S. Kim, J.-K. Min, D. Kim, and J. Jung
A Soluble Auxin-binding Protein, ABP57. PURIFICATION WITH ANTI-BOVINE SERUM ALBUMIN ANTIBODY AND CHARACTERIZATION OF ITS MECHANISTIC ROLE IN THE AUXIN EFFECT ON PLANT PLASMA MEMBRANE H+-ATPase
J. Biol. Chem., March 30, 2001; 276(14): 10730 - 10736.
[Abstract] [Full Text] [PDF]


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Copyright © 1985 by the American Society of Plant Biologists