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Plant Physiology 79:1090-1093 (1985)
© 1985 American Society of Plant Biologists

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Hydrolysis of Intracellular Proteins in Vacuoles Isolated from Acer pseudoplatanus L. Cells 1

Hervé Canut, Gilbert Alibert and Alain M. Boudet

Centre de Physiologie Végétale de l'Université Paul Sabatier, Unité Associée au Centre National de la Recherche Scientifique, No. 241, 118 Route de Narbonne, F-31062 Toulouse, Cédex, France

Acer pseudoplatanus cell suspension cultures were used to examine the ability of vacuoles isolated from protoplasts to hydrolyze their endogenous proteins. Total cell proteins were labeled by addition of [3H]leucine to the culture medium. After preparation of the protoplasts, vacuoles were isolated and were shown to be essentially free from other cellular components. Up to 30% of the [3H]leucine-labeled newly synthesized proteins were recovered in the vacuoles. When incubated for 6 hours at 20°C, the vacuoles degraded half of these proteins. The protein breakdown was temperature and pH dependent. Analysis by electrophoresis, in denaturing polyacrylamide gels, revealed that most of the vacuolar proteins were degraded. However, some vacuolar proteins were unaffected during a 6-hour incubation period. The results indicate that vacuoles are able to acquire and degrade intracellular proteins.

1 Supported by grants from the Centre National de la Recherche Scientifique and from the Université Paul Sabatier Toulouse.




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Copyright © 1985 by the American Society of Plant Biologists