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Plant Physiology 80:14-19 (1986)
© 1986 American Society of Plant Biologists

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Articles

13C6-[Benzene Ring]-Indole-3-Acetic Acid

A New Internal Standard for Quantitative Mass Spectral Analysis of Indole-3-Acetic Acid in Plants

Jerry D. Cohen, Bruce G. Baldi1 and Janet Pernise Slovin

United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705, Plant Hormone Laboratory, Beltsville Agricultural Research Center-West, Beltsville, Maryland 20705

Indole-3-acetic acid (IAA) labeled with 13C in the six carbons of the benzene ring is described for use as an internal standard for quantitative mass spectral analysis of IAA by gas chromatography/selected ion monitoring. [13C6]IAA was compared to the available deuterium labeled compounds and shown to offer the advantages of nonexchangeability of the isotope label, high isotopic enrichment, and chromatographic properties identical to that of the unlabeled compound. The utility of [13C6]IAA for measurement of endogenous IAA levels was demonstrated by analysis of IAA in Lemna gibba G-3.

1 Current address: Institute of Biological Chemistry, Washington State University, Pullman, WA 99164.




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