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Plant Physiology 80:390-395 (1986)
© 1986 American Society of Plant Biologists

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Articles

D-Glycerate Transport by the Pea Chloroplast Glycolate Carrier 1

Studies on [1-14C]D-Glycerate Uptake and D-Glycerate Dependent O2 Evolution

Konrad T. Howitz and Richard E. McCarty

Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853

The transport of glycolate and D-glycerate, the substrate and end product of the photorespiratory carbon pathway, respectively, by isolated intact pea (Pisum sativum) chloroplasts has been compared. D-Glycerate uptake was inhibited by the 2-hydroxymonocarboxylates, glycolate, glyoxylate, and D-lactate. Phosphate and phosphoglycerate and triose phosphates were without effect when the assays were carried out for two seconds. Glycolate was found to be a competitive inhibitor of D-glycerate uptake and the presence of glycolate in the chloroplast stroma strongly enhanced D-glycerate uptake from the medium. For optimal rates of D-glycerate-dependent O2 evolution by pea chloroplasts, phosphate, ADP or ATP, and a mediator of cyclic electron flow had to be added. The inhibition of D-glycerate-dependent O2 evolution by triose phosphates and 2-hydroxymonocarboxylates was tested. The inhibition of D-glycerate-dependent O2 evolution by these metabolites did not correlate with their effects on glycerate transport. Thus, metabolism of D-glycerate, rather than its transport, limits the rate of glycerate-dependent oxygen evolution. The ramifications of D-glycerate metabolism on the interpretation of D-glycerate uptake data obtained with prolonged incubations are discussed. We conclude that D-glycerate and glycolate transport are mediated by the same transport system.

1 Supported by a grant 83-00670 from the Competitive Research Grants Office of the United States Department of Agriculture.






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Copyright © 1986 by the American Society of Plant Biologists