This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (146) Citing Articles via Google Scholar Google Scholar Articles by Kombrink, E. Articles by Hahlbrock, K. Search for Related Content PubMed PubMed Citation Articles by Kombrink, E. Articles by Hahlbrock, K. Agricola Articles by Kombrink, E. Articles by Hahlbrock, K.

Articles

Responses of Cultured Parsley Cells to Elicitors from Phytopathogenic Fungi ¹

Timing and Dose Dependency of Elicitor-Induced Reactions

Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, 5000 Köln 30, Federal Republic of Germany

Cultured parsley cells (Petroselinum crispum) responded to treatment with heat-released soluble cell-wall fragments (elicitors) from several different phytopathogenic fungi by forming coumarin derivatives (phytoalexins). This response was preceded in all cases by large but transient increases in the activities of two enzymes of general phenylpropanoid metabolism, phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL). The activities of two hydrolytic enzymes, chitinase and 1,3--glucanase, also increased strongly in elicitor-treated cells, whereas the activities of three enzymes participating in primary metabolism were affected differently by the elicitor treatment. Glucose-6-phosphate dehydrogenase increased, phosphofructokinase remained almost constant, and pyrophosphate:fructose-6-phosphate phosphotransferase declined sharply in activity. Different amounts of cell-wall preparations from various phytopathogenic fungi were required for maximum elicitor activity. While three oomycetes (Phytophthora spp.) yielded the most active elicitors studied (maximum coumarin accumulation at concentrations of about 10 microgram per milliliter), cell-wall preparations from an ascomycete and three deuteromycetes gave comparable results only at 10 to 100 times higher concentrations. Optimal induction of PAL, 4CL, and chitinase with Phytophthora elicitor required only about 1 microgram per milliliter, whereas 1,3--glucanase induction showed a dose dependence similar to that observed for coumarins. The elicitor concentration had pronounced effects not only on the extent, but also on the timing of all induced reactions.

This article has been cited by other articles:

				F. Turck, A. Zhou, and I. E. Somssich Stimulus-Dependent, Promoter-Specific Binding of Transcription Factor WRKY1 to Its Native Promoter and the Defense-Related Gene PcPR1-1 in Parsley PLANT CELL, October 1, 2004; 16(10): 2573 - 2585. [Abstract] [Full Text] [PDF]

				R. GOMEZ-VASQUEZ, R. DAY, H. BUSCHMANN, S. RANDLES, J. R. BEECHING, and R. M. COOPER Phenylpropanoids, Phenylalanine Ammonia Lyase and Peroxidases in Elicitor-challenged Cassava (Manihot esculenta) Suspension Cells and Leaves Ann. Bot., July 1, 2004; 94(1): 87 - 97. [Abstract] [Full Text] [PDF]

				A. Heise, B. Lippok, C. Kirsch, and K. Hahlbrock Two immediate-early pathogen-responsive members of the AtCMPG gene family in Arabidopsis thaliana and the W-box-containing elicitor-response element of AtCMPG1 PNAS, June 25, 2002; 99(13): 9049 - 9054. [Abstract] [Full Text] [PDF]

				E. Logemann, A. Tavernaro, W. Schulz, I. E. Somssich, and K. Hahlbrock UV light selectively coinduces supply pathways from primary metabolism and flavonoid secondary product formation in parsley PNAS, February 15, 2000; 97(4): 1903 - 1907. [Abstract] [Full Text] [PDF]

				R. S. Redman, S. Freeman, D. R. Clifton, J. Morrel, G. Brown, and R. J. Rodriguez Biochemical Analysis of Plant Protection Afforded by a Nonpathogenic Endophytic Mutant of Colletotrichum magna Plant Physiology, February 1, 1999; 119(2): 795 - 804. [Abstract] [Full Text]

				E. Koopmann, E. Logemann, and K. Hahlbrock Regulation and Functional Expression of Cinnamate 4-Hydroxylase from Parsley Plant Physiology, January 1, 1999; 119(1): 49 - 56. [Abstract] [Full Text]

				S. Gus-Mayer, B. Naton, K. Hahlbrock, and E. Schmelzer Local mechanical stimulation induces components of the pathogen defense response in parsley PNAS, July 7, 1998; 95(14): 8398 - 8403. [Abstract] [Full Text] [PDF]

				E. Koopmann and K. Hahlbrock Differentially regulated NADPH:cytochrome P450 oxidoreductases in parsley PNAS, December 23, 1997; 94(26): 14954 - 14959. [Abstract] [Full Text] [PDF]

				T. Jabs, M. Tschope, C. Colling, K. Hahlbrock, and D. Scheel Elicitor-stimulated ion fluxes and O2- from the oxidative burst are essential components in triggering defense gene activation and phytoalexin synthesis in parsley PNAS, April 29, 1997; 94(9): 4800 - 4805. [Abstract] [Full Text] [PDF]

				S. Zimmermann, T. Nurnberger, J.-M. Frachisse, W. Wirtz, J. Guern, R. Hedrich, and D. Scheel Receptor-mediated activation of a plant Ca2+-permeable ion channel involved in pathogen defense PNAS, March 18, 1997; 94(6): 2751 - 2755. [Abstract] [Full Text] [PDF]

				C. Kirsch, M. Takamiya-Wik, S. Reinold, K. Hahlbrock, and I. E. Somssich Rapid, transient, and highly localized induction of plastidial omega -3 fatty acid desaturase mRNA at fungal infection sites in Petroselinum crispum PNAS, March 4, 1997; 94(5): 2079 - 2084. [Abstract] [Full Text] [PDF]