This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (117) Citing Articles via Google Scholar Google Scholar Articles by An, G. Articles by Chiang, C. C. Search for Related Content PubMed PubMed Citation Articles by An, G. Articles by Chiang, C. C. Agricola Articles by An, G. Articles by Chiang, C. C.

Articles

Transformation of Tobacco, Tomato, Potato, and Arabidopsis thaliana Using a Binary Ti Vector System ¹

Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340

Using a binary tumor-inducing (Ti) plasmid vector system, several plant species were transformed with a kanamycin resistance marker (neomycin phosphotransferase gene). Four Nicotiana species, seven tomato cultivars, two potato cultivars, and Arabidopsis thaliana were transformed by the binary vector transformation method. In this method, various plant organ pieces were co-cultivated with Agrobacterium tumefaciens cells carrying the binary vector, pGA472, and a helper Ti plasmid. We have also demonstrated that a wild type Ti plasmid can be used as a helper to obtain a transformed plant.

This article has been cited by other articles:

				C.-C. Chang, P.-S. Huang, H.-R. Lin, and C.-H. Lu Transactivation of Protein Expression by Rice HSP101 in Planta and Using Hsp101 as a Selection Marker for Transformation Plant Cell Physiol., August 1, 2007; 48(8): 1098 - 1107. [Abstract] [Full Text] [PDF]

				S. Kitakura, T. Fujita, Y. Ueno, S. Terakura, H. Wabiko, and Y. Machida The Protein Encoded by Oncogene 6b from Agrobacterium tumefaciens Interacts with a Nuclear Protein of Tobacco PLANT CELL, February 1, 2002; 14(2): 451 - 463. [Abstract] [Full Text] [PDF]

				R. S. Lewis and E. A. Wernsman Efforts to Initiate Construction of a Disease Resistance Package on a Designer Chromosome in Tobacco Crop Sci., September 1, 2001; 41(5): 1420 - 1427. [Abstract] [Full Text] [PDF]

				E. M. Meyerowitz Prehistory and History of Arabidopsis Research Plant Physiology, January 1, 2001; 125(1): 15 - 19. [Full Text]

				Y.-G. Liu, Y. Shirano, H. Fukaki, Y. Yanai, M. Tasaka, S. Tabata, and D. Shibata Complementation of plant mutants with large genomic DNA fragments by a transformation-competent artificial chromosome vector accelerates positional cloning PNAS, May 25, 1999; 96(11): 6535 - 6540. [Abstract] [Full Text] [PDF]

				D. W. Meinke, J. M. Cherry, C. Dean, S. D. Rounsley, and M. Koornneef Arabidopsis thaliana: A Model Plant for Genome Analysis Science, October 23, 1998; 282(5389): 662 - 682. [Abstract] [Full Text]

				P Dozolme, D Marty-Mazars, M. Clemencet, and F Marty Monoclonal antibody TeM 106 reacts with a tonoplast intrinsic protein of 106 kDa from Brassica oleracea L J. Cell Sci., January 4, 1995; 108(4): 1509 - 1517. [Abstract] [PDF]