Plant Physiol.
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Plant Physiology 81:8-11 (1986)
© 1986 American Society of Plant Biologists

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Articles

Amino Acid Transport in Protoplasts Isolated from Soybean Leaves 1

Charles D. Vernooy and Willy Lin

Central Research and Development Department, Experimental Station, E. I. du Pont de Nemours & Company, Wilmington, Delaware 19898

We isolated large quantities of mesophyll protoplasts from source and sink leaves of soybean plants and examined them for amino acid uptake. Accumulation of amino acids in isolated protoplasts was linear for at least 40 minutes. Uptake kinetics revealed the presence of both saturable and linear components. Increasing external pH decreases the uptake. The uncoupler, carbonyl cyanide p-trifluoromethoxyphenylhydrazone at 15 micromolar inhibited and fusicoccin at 10 micromolar stimulated amino acid uptake. Our data are consistent with a proton-cotransport mechanism for the uptake of L-glutamine and {alpha}-amino isobutyric acid into soybean mesophyll cells.

1 Contribution No. 3794 from the Central Research and Development Department, Experimental Station, E. I. du Pont de Nemours & Company, Wilmington, DE 19898.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1986 by the American Society of Plant Biologists