Plant Physiol. Drug Metab Dispos
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Plant Physiology 81:854-858 (1986)
© 1986 American Society of Plant Biologists

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Articles

Assay of Photosynthetic Oxygen Evolution from Single Protoplasts 1,2

Rüdiger Hampp, Werner Mehrle and Ulrich Zimmermann

Biologie I, Universität Tübingen, D-7400 Tübingen 1, Federal Republic of Germany, Institut für Biotechnologie, Universität Würzburg, D-8700 Würzburg, Federal Republic of Germany

A semiquantitative assay for light-dependent O2 evolution by a single mesophyll protoplast is described. The assay indicator is the density of aerotactic bacteria (Pseudomonas aeruginosa, ATCC 10145; `Engelmann experiment') attracted to the protoplast. Quantification is by dark field microphotometry. The sensitivity is about 50 femtomoles O2 per protoplast per minute. The results demonstrate the biphasic nature of O2 evolution of a single protoplast during photosynthetic induction. Computerized data acquisition yields traces which, until a steady state of photosynthetic O2 evolution is reached, are identical to ordinary O2 electrode traces.

1 Dedicated to Prof. E. Bünning on the occasion of his 80th birthday.

2 Supported by grants from the Deutsche Forschungsgemeinschaft to R. H. and the Bundesministerium für Forschung und Technologie to U. Z (no. 0373509).






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Copyright © 1986 by the American Society of Plant Biologists