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Articles

Metabolism under Microaerobic Conditions of Mitochondria from Cowpea Nodules ¹

Boyce Thompson Institute, Tower Road, Ithaca, New York 14853

A method is described for isolating mitochondria from nodules of cowpea (Vigna unguiculata [L.] Walp.) under completely anaerobic conditions. The mitochondria were immediately active when incubated aerobically with substrates, and their respiration rates were higher than mitochondria prepared in air. The mitochondria lacked fumarate reductase and were not inhibited by 5% CO₂. When incubated under microaerobic conditions, their respiration could be measured by leghemoglobin spectroscopy. Microaerobic respiration was inhibited approximately 50% by 1 millimolar malonate, and was completely inhibited by cyanide. O₂ uptake and the ATP/O ratio declined under microaerobic conditions, and therefore ATP production may be low in the environment of infected nodule cells.

¹ Supported by contract 82ER-12066 from the United States Department of Energy, Division of Biological Energy Sciences.

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