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Plant Physiology 81:976-979 (1986) © 1986 American Society of Plant Biologists Phototropism in Hypocotyls of Radish 1I. Isolation and Identification of Growth Inhibitors, cis- and trans-Raphanusanins and Raphanusamide, Involved in Phototropism of Radish HypocotylsBiological Institute, College of Liberal Arts, Kagoshima University, Korimoto 1-21-30, Kagoshima 890, Japan, Department of Chemistry, Faculty of Science, Kagoshima University, Korimoto 1-21-30, Kagoshima 890, Japan
Three growth inhibitors which might be involved in phototropism of Sakurajima radish (Raphanus sativus var. hortensis f. gigantissimus Makino) hypocotyls, were isolated as crystalline forms from light-exposed radish seedlings and identified as cis- and trans-raphanusanins and 6-methoxy-2,3,4,5-tetrahydro-1,3-oxazepin-2-one (designated raphanusamide). The cis- and trans-raphanusanins inhibited growth of etiolated radish hypocotyls at concentrations higher than 1.5 micromolar, raphanusamide at concentrations higher than 20 micromolar.
1 Partly supported by grants-in-aid for Scientific Research in 1983 to 1985 NO. 58540430 to K. H. from the Japanese Ministry of Education, Science and Culture.
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