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Plant Physiology 82:218-221 (1986)
© 1986 American Society of Plant Biologists

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Articles

Opine Synthesis in Wild-Type Plant Tissue

Paul Christou, Steven G. Platt and Mary C. Ackerman

Agracetus, 8520 University Green, Middleton, Wisconsin 53562

Opine production is associated with crown gall tissue, a neoplastic growth caused by infection of dicotyledonous plants with Agrobacterium tumefaciens. Recent publications have claimed that tissues of certain monocotyledonous plants can also be infected by Agrobacterium. Following infection, a part of the Agrobacterium Ti plasmid, T-DNA, is integrated into the chromosome of the infected plant. T-DNA, which codes for opine-synthesizing enzymes, is now used to add foreign genes to plants. A number of laboratories have used opine production in plant tissue, often after arginine feeding or preincubation as evidence for plant transformation by T-DNA vectors. In this report we provide microbiological, chromatographic, spectroscopic and chemical evidence indicating that opines can be formed in normal callus and plant tissue as a result of arginine metabolism. Therefore, researchers studying T-DNA should be aware of the capability of plant tissue to metabolize arginine to opines. Opine production following infection with T-DNA may not always be sufficient evidence to indicate transformation by the Agrobacterium Ti plasmid.




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E. C. COCKING and M. R. DAVEY
Gene Transfer in Cereals
Science, June 5, 1987; 236(4806): 1259 - 1262.
[Abstract] [PDF]


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Copyright © 1986 by the American Society of Plant Biologists