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Plant Physiology 82:339-345 (1986)
© 1986 American Society of Plant Biologists

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Articles

The Occurrence of Abscisic Acid and Abscisyl-{beta}-D-Glucopyranoside in Developing and Mature Citrus Fruit as Determined by Enzyme Immunoassay 1

Michael J. Harris2 and William M. Dugger

Department of Botany and Plant Sciences, University of California, Riverside, California 92521

The contents of (+)-cis-abscisic acid (ABA) and alkaline-hydrolyzable ABA-conjugate(s) were analyzed by means of enzyme immunoassay in partially purified extracts of developing and mature sweet orange fruit (Citrus sinensis [L.] Osbeck cv Washington navel). A relatively small increase in ABA was observed in the fruit exocarp during the natural color transition from green to orange. At the same time, the ABA-conjugate level increased approximately 12-fold in this tissue. The contents of ABA and ABA-conjugate equaled 15.0 ± 0.7 and 107.8 ± 2.1 nanomoles per gram fresh weight, respectively, in the exocarp at harvest. Other tissues also contained considerable quantities of these compounds. Whereas the highest ABA content was observed in the exocarp, the highest ABA-conjugate content was observed in the central vascular axis of the fruit and equaled 187.0 ± 10.3 nanomoles per gram fresh weight. The only immunoreactive conjugate found in significant quantity in mature fruit was identified as abscisyl-{beta}-D-glucopyranoside (ABA-GE) based on (a) immunological cross-reactivity, (b) thin layer chromatography co-chromatography with authentic standards in two solvent systems, (c) susceptibility to both chemical and enzymic degradation, and (d) mass spectroscopy.

2 Present address: Department of Biological Sciences, Florida State University, Tallahassee, FL.

1 Supported from funds provided by the University of California Agricultural Experiment Station.




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M. Kato, H. Matsumoto, Y. Ikoma, H. Okuda, and M. Yano
The role of carotenoid cleavage dioxygenases in the regulation of carotenoid profiles during maturation in citrus fruit
J. Exp. Bot., July 1, 2006; 57(10): 2153 - 2164.
[Abstract] [Full Text] [PDF]


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Copyright © 1986 by the American Society of Plant Biologists