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Plant Physiology 82:396-400 (1986)
© 1986 American Society of Plant Biologists

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Regulation of Plasma Membrane beta-Glucan Synthase from Red Beet Root by Phospholipids 1

Reactivation of Triton X-100 Extracted Glucan Synthase by Phospholipids

Bruce P. Wasserman and Kevin J. McCarthy

Department of Food Science, New Jersey Agricultural Experiment Station, Cook College, Rutgers University, New Brunswick, New Jersey 08903

Extraction of red beet root plasma membranes with the detergent Triton X-100 at a level of 2.0% (weight/volume) resulted in the depletion of over 90% of total membrane phospholipid and the reduction of glucan synthase activity by 80 to 90%. Reconstitution of the delipidated Triton X-100, 100,000g fraction in the presence of phospholipids restored glucan synthase activity. The most effective phospholipid was phosphatidyl-ethanolamine, which restored 110 to 144% of the original activity at 0.5% (weight/volume). Glucan synthase in the phospholipid-reactivated Triton X-100-treated fraction was enriched 9-fold in specific activity relative to microsomal membranes but was unstable in digitonin. These results support the hypothesis that glucan synthase activity is regulated by its phospholipid environment.

1 Supported by grant DMB 85-02523 from the National Science Foundation, a Biomedical Research Support Grant and the New Jersey Agricultural Experiment Station with State and Hatch Act Funds. New Jersey Agricultural Experiment Station, Publication No. D-10538-2-86.




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F. Goubet and D. Mohnen
Solubilization and Partial Characterization of Homogalacturonan-Methyltransferase from Microsomal Membranes of Suspension-Cultured Tobacco Cells
Plant Physiology, September 1, 1999; 121(1): 281 - 290.
[Abstract] [Full Text]


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Copyright © 1986 by the American Society of Plant Biologists