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Articles

Oxygen Isotope Exchange between Metabolites and Water during Biochemical Reactions Leading to Cellulose Synthesis ¹

Department of Earth and Space Sciences, University of California, Los Angeles, California 90024, Department of Forest Resources, University of New Brunswick, Bag Service Number 44555, Fredericton, N.B. E3B 6C2

Cellulose was produced heterotrophically from different carbon substrates by carrot tissue cultures and Acetobacter xylinum (a cellulose-producing bacterium) and by castor bean seeds germinated in the dark, in each case in the presence of water having known concentration of oxygen-18 (¹⁸O). We used the relationship between the amount of ¹⁸O in the water and in the cellulose that was synthesized to determine the number and ¹⁸O content of the substrate oxygens that exchanged with water during the reactions leading to cellulose synthesis. Our observations support the hypothesis that oxygen isotope ratios of plant cellulose are determined by isotopic exchange occurring during hydration of carbonyl groups of the intermediates of cellulose synthesis.

³ Also in Archaeology Program.

¹ Supported in part by National Science Foundation grant DMB 84-05003.

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