Plant Physiol. Illumina
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Plant Physiology 82:454-456 (1986)
© 1986 American Society of Plant Biologists

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Articles

Accumulation of 14C-Radiolabel in Leaves and Fruits after Injection of [14C]Tryptophan into Seeds of Soybean 1

Mich B. Hein2, Mark L. Brenner and William A. Brun

Department of Horticultural Science and Landscape Architecture, University of St. Paul, St. Paul, Minnesota 55108, Department of Agronomy and Plant Genetics, University of St. Paul, St. Paul, Minnesota 55108

Injection of [14C]tryptophan into one seed in a soybean fruit resulted in recovery of radiolabel in a fraction that cochromatographed with indoleacetic acid (IAA) through three successive high performance liquid chromatography separations. Label was found in the putative IAA fraction in all of the fruit tissues sampled and in the blade of the leaf subtending the pod into which the radiolabeled tryptophan had been injected. This suggested that IAA or an IAA precursor was transported from seeds to other parts of the fruit and to subtending leaves.

2 Present address: Research Institute of the Scripps Clinic, Dept. of Molecular Biology, MB-3, 10666 N. Torrey Pine Rd., LaJolla, CA 92037.

1 Supported in part by the United States Department of Agricultrue under grant 5901-0410-8-0183-0 from the Competitive Research Grants Office. Also supported in part by a grant from the Minnesota Soybean Research and Promotion Council. Contribution from the University of Minnesota Agricultural Experiment Station. St. Paul, MN 55108. Paper No. 14,139 Journal Series.






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Copyright © 1986 by the American Society of Plant Biologists