Plant Physiol. Illumina
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Plant Physiology 82:771-779 (1986)
© 1986 American Society of Plant Biologists

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Articles

A System for Manipulating the Membrane Fatty Acid Composition of Soybean Cell Cultures by Adding Tween-Fatty Acid Esters to Their Growth Medium 1

Basic Parameters and Effects on Cell Growth

William B. Terzaghi2

Department of Biology, University of Utah, Salt Lake City, Utah 84112

The development of a system for modifying the membrane fatty acid composition of cultured soybean cells (Glycine max [L.] Merr.) is described. Tween-fatty acid esters carrying specific fatty acids were synthesized and added to the medium of suspension cultures. Cells transferred large quantities of exogenous fatty acids from Tweens to all acylated membrane lipids; up to 50% of membrane fatty acids were exogenously derived. C15 to C20 saturated fatty acids and C16, C18, and C20 unsaturated fatty acids with either cis or trans double bonds were incorporated into lipids. Cells elongated saturated fatty acids of C16 or less, and unsaturated fatty acids with cis double bonds were further desaturated. No other types of modifications were observed. Growth ceased in cells treated with excessive concentrations of Tween-fatty acid esters, but frequently not for several days. Cessation of cell growth was correlated with changes in membrane fatty acid composition resulting from incorporation of large amounts of exogenous fatty acids into membrane lipids, although cells tolerated large variations in fatty acid composition. Maximum tolerable Tween concentrations varied widely according to the fatty acid supplied. Potential uses of this system and implications of the observed modifications on the pathway of incorporation are discussed.

2 Supported by National Science Foundation predoctoral fellowship SPE 835-0132 and by a University of Utah graduate research fellowship. Present address: Carnegie Institution of Washington, Department of Plant Biology, Stanford CA 94305.

1 Supported by National Institute of Environmental Health Sciences grant 01498 to Dr. Karl G. Lark. This research was conducted in partial fulfillment of the doctoral degree requirement of the Department of Biology, University of Utah.






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ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1986 by the American Society of Plant Biologists