Plant Physiology 82:787-794 (1986)
© 1986 American Society of Plant Biologists
Articles
Immunogold Localization of Xyloglucan and Rhamnogalacturonan I in the Cell Walls of Suspension-Cultured Sycamore Cells 1
Patricia J. Moore,
Alan G. Darvill2,
Peter Albersheim2 and
L. Andrew Staehelin
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347
Plant cell walls serve several functions: they impart rigidity to the plant, provide a physical and chemical barrier between the cell and its environment, and regulate the size and shape of each cell. Chemical studies have provided information on the biochemical composition of the plant cell walls as well as detailed knowledge of individual cell wall molecules. In contrast, very little is known about the distribution of specific cell wall components around individual cells and throughout tissues. To address this problem, we have produced polyclonal antibodies against two cell wall matrix components; rhamnogalacturonan I (RG-I), a pectic polysaccharide, and xyloglucan (XG), a hemicellulose. By using the antibiodies as specific markers we have been able to localize these polymers on thin sections of suspension-cultured sycamore cells (Acer pseudoplatanus). Our results reveal that each molecule has a unique distribution. XG is localized throughout the entire wall and middle lamella. RG-I is restricted to the middle lamella and is especially evident in the junctions between cells. These observations indicate that plant cell walls may have more distinct chemical (and functional?) domains than previously envisaged.
2 Present address: Complex Carbohydrate Research Center, Richard B. Russel Agricultural Research Center, Athens, GA 30613.
1 Supported by National Institutes of Health-GM 18639 to L. A. S., Department of Energy number DE-FG09-85-ER13426 to P. A., and National Science Foundation DMBB85-45798 to A.G.D.
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