Plant Physiology 82:1024-1030 (1986)
© 1986 American Society of Plant Biologists
Articles
Accumulation and Conversion of Sugars by Developing Wheat Grains 1
VI. Gradients Along the Transport Pathway from the Peduncle to the Endosperm Cavity during Grain Filling
Donald B. Fisher2 and
Roger M. Gifford
Department of Botany, Washington State University, Pullman, Washington 99164,
Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, G.P.O. Box 1600, Canberra, A.C.T. 2601, Australia
Gradients along the transport pathway from the peduncle to the endosperm cavity were examined during grain filling in wheat. Sieve tube exudate was collected from severed aphid stylets established on the peduncle and rachis and on the vascular bundles in the creases of grains. Phloem exudate could also be collected from broken grain pedicels, and by puncturing the vascular bundle in the grain crease with a needle. Stylets on excised grains persisted exuding, indicating that grain sieve tubes are capable of loading solutes. There was little, if any, discernible gradient in osmolality or solute composition (sucrose, total amino acids) of sieve tube contents along the phloem pathway from the peduncle to the rachis or along the rachis itself. Neither was a gradient detected in osmolality along the sieve tube pathway from the rachis through the rachilla and grain stalk to the crease. Demonstrable solute gradients occurred only across those tissues of the grain crease between the crease sieve tubes and the endosperm cavity, a distance of just 1 millimeter. However, while the sucrose concentration in the sieve tubes was almost tenfold that in the endosperm cavity sap, total amino acids were only threefold higher, and the potassium concentrations of the two were equal. Our observations strongly implicate the movement of assimilates from the sieve tubes and across the crease tissues as important control points in grain filling.
2 Visiting Scientist at the Commonwealth Scientific and Industrial Research Organization, Division of Plant Industry under the United States/Australia Cooperative Science Program.
1 Supported by National Science Foundation grant PCM 83-04693.
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