Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 83:155-158 (1987)
© 1987 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (187)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wallsgrove, R. M.
Right arrow Articles by Bright, S. W. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wallsgrove, R. M.
Right arrow Articles by Bright, S. W. J.
Agricola
Right arrow Articles by Wallsgrove, R. M.
Right arrow Articles by Bright, S. W. J.
Metabolism and Enzymology

Barley Mutants Lacking Chloroplast Glutamine Synthetase—Biochemical and Genetic Analysis

Roger M. Wallsgrove, Janice C. Turner, Nigel P. Hall, Alan C. Kendall and Simon W. J. Bright

Biochemistry Department, Rothamsted Experimental Station, Harpenden, Hertfordshire AL5 2JQ, United Kingdom

Eight mutants of barley (Hordeum vulgare cv Maris Mink) lacking the chloroplast isozyme of glutamine synthetase (EC 6.3.1.2.) were isolated by their inability to grow under photorespiratory conditions. The cytoplasmic isozyme of glutamine synthetase was present in the leaves of all the mutants, with activities comparable to the wild-type (10-12 nanokatals per gram fresh weight). The mutant plants developed normally and were fully fertile under conditions that minimize photorespiration. In 1% O2 the rate of CO2 fixation in leaves of one of the mutants, RPr 83/32, was the same as the wild-type, but in air this rate declined to 60% of the wild-type after 30 minutes. During this time the ammonia concentration in leaves of the mutant rose from 1 to 50 micromoles per gram fresh weight. Such ammonia accumulation in air was found in all the mutant lines. In back-crosses with the parent line, F1 plants were viable in air. In the F2 generation, nonviability in air and the lack of chloroplast glutamine synthetase co-segregated, in both the lines tested. These two lines and four others proved to be allelic; we designate them gln 2a-f. The characteristics of these mutants conclusively demonstrate the major role of chloroplast glutamine synthetase in photorespiration and its associated nitrogen recycling.




This article has been cited by other articles:


Home page
 J Exp BotHome page
M. Tabuchi, T. Abiko, and T. Yamaya
Assimilation of ammonium ions and reutilization of nitrogen in rice (Oryza sativa L.)
J. Exp. Bot., July 1, 2007; 58(9): 2319 - 2327.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. P. Purnell and J. R. Botella
Tobacco Isoenzyme 1 of NAD(H)-Dependent Glutamate Dehydrogenase Catabolizes Glutamate in Vivo
Plant Physiology, January 1, 2007; 143(1): 530 - 539.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Lima, A. Seabra, P. Melo, J. Cullimore, and H. Carvalho
Post-translational regulation of cytosolic glutamine synthetase of Medicago truncatula
J. Exp. Bot., August 1, 2006; 57(11): 2751 - 2761.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Ishiyama, E. Inoue, T. Yamaya, and H. Takahashi
Gln49 and Ser174 Residues Play Critical Roles in Determining the Catalytic Efficiencies of Plant Glutamine Synthetase
Plant Cell Physiol., February 1, 2006; 47(2): 299 - 303.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
J. Teixeira, S. Pereira, F. Canovas, and R. Salema
Glutamine synthetase of potato (Solanum tuberosum L. cv. Desiree) plants: cell- and organ-specific expression and differential developmental regulation reveal specific roles in nitrogen assimilation and mobilization
J. Exp. Bot., February 1, 2005; 56(412): 663 - 671.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K. Ishiyama, E. Inoue, M. Tabuchi, T. Yamaya, and H. Takahashi
Biochemical Background and Compartmentalized Functions of Cytosolic Glutamine Synthetase for Active Ammonium Assimilation in Rice Roots
Plant Cell Physiol., November 15, 2004; 45(11): 1640 - 1647.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. Larios, E. Aguera, P. Cabello, J. M. Maldonado, and P. de la Haba
The rate of CO2 assimilation controls the expression and activity of glutamine synthetase through sugar formation in sunflower (Helianthus annuus L.) leaves
J. Exp. Bot., January 1, 2004; 55(394): 69 - 75.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Kang and F. J. Turano
The putative glutamate receptor 1.1 (AtGLR1.1) functions as a regulator of carbon and nitrogen metabolism in Arabidopsis thaliana
PNAS, May 27, 2003; 100(11): 6872 - 6877.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. M. Melo, L. M. Lima, I. M. Santos, H. G. Carvalho, and J. V. Cullimore
Expression of the Plastid-Located Glutamine Synthetase of Medicago truncatula. Accumulation of the Precursor in Root Nodules Reveals an in Vivo Control at the Level of Protein Import into Plastids
Plant Physiology, May 1, 2003; 132(1): 390 - 399.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Sohlenkamp, C. C. Wood, G. W. Roeb, and M. K. Udvardi
Characterization of Arabidopsis AtAMT2, a High-Affinity Ammonium Transporter of the Plasma Membrane
Plant Physiology, December 1, 2002; 130(4): 1788 - 1796.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Husted, M. Mattsson, C. Mollers, M. Wallbraun, and J. K. Schjoerring
Photorespiratory NH4+ Production in Leaves of Wild-Type and Glutamine Synthetase 2 Antisense Oilseed Rape
Plant Physiology, October 1, 2002; 130(2): 989 - 998.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
I. C. Oliveira, T. Brears, T. J. Knight, A. Clark, and G. M. Coruzzi
Overexpression of Cytosolic Glutamine Synthetase. Relation to Nitrogen, Light, and Photorespiration
Plant Physiology, July 1, 2002; 129(3): 1170 - 1180.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Weber and U.-I. Flugge
Interaction of cytosolic and plastidic nitrogen metabolism in plants
J. Exp. Bot., April 15, 2002; 53(370): 865 - 874.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Chichkova, J. Arellano, C. P. Vance, and G. Hernandez
Transgenic tobacco plants that overexpress alfalfa NADH-glutamate synthase have higher carbon and nitrogen content
J. Exp. Bot., November 1, 2001; 52(364): 2079 - 2087.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. I. Fuentes, D. J. Allen, A. Ortiz-Lopez, and G. Hernandez
Over-expression of cytosolic glutamine synthetase increases photosynthesis and growth at low nitrogen concentrations
J. Exp. Bot., May 1, 2001; 52(358): 1071 - 1081.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. H. Roosens, R. Willem, Y. Li, I. Verbruggen, M. Biesemans, and M. Jacobs
Proline Metabolism in the Wild-Type and in a Salt-Tolerant Mutant of Nicotiana plumbaginifolia Studied by 13C-Nuclear Magnetic Resonance Imaging
Plant Physiology, December 1, 1999; 121(4): 1281 - 1290.
[Abstract] [Full Text]

Home page
 Plant CellHome page
N. Brugière, F. Dubois, A. M. Limami, M. Lelandais, Y. Roux, R. S. Sangwan, and B. Hirel
Glutamine Synthetase in the Phloem Plays a Major Role in Controlling Proline Production
PLANT CELL, October 1, 1999; 11(10): 1995 - 2012.
[Abstract] [Full Text]

Home page
 Plant CellHome page
S. Gazzarrini, L. Lejay, A. Gojon, O. Ninnemann, W. B. Frommer, and N. von Wirén
Three Functional Transporters for Constitutive, Diurnally Regulated, and Starvation-Induced Uptake of Ammonium into Arabidopsis Roots
PLANT CELL, May 1, 1999; 11(5): 937 - 948.
[Abstract] [Full Text]

Home page
 GeneticsHome page
C. J. Schultz, M. Hsu, B. Miesak, and G. M. Coruzzi
Arabidopsis Mutants Define an in Vivo Role for Isoenzymes of Aspartate Aminotransferase in Plant Nitrogen Assimilation
Genetics, June 1, 1998; 149(2): 491 - 499.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. T. Coschigano, R. Melo-Oliveira, J. Lim, and G. M. Coruzzi
Arabidopsis gls Mutants and Distinct Fd-GOGAT Genes: Implications for Photorespiration and Primar y Nitrogen Assimilation
PLANT CELL, May 1, 1998; 10(5): 741 - 752.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1987 by the American Society of Plant Biologists