Plant Physiol. Illumina
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Plant Physiology 83:229-231 (1987)
© 1987 American Society of Plant Biologists

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Molecular Biology and Gene Regulation

5-Enolpyruvylshikimate 3-Phosphate Synthase, the Target Enzyme of the Herbicide Glyphosate, Is Synthesized as a Precursor in a Higher Plant 1

Heike Holländer-Czytko and Nikolaus Amrhein

Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, D 4630 Bochum, Federal Republic of Germany

Cell cultures of Corydalis sempervirens adapted to growth in the presence of 5 millimolar glyphosate overproduce the herbicide's target enzyme, 5-enolpyruvylshikimate 3-phosphate (EPSP) synthase, 30- to 40-fold. In vitro translation of total RNA and poly(A)-RNA coupled with immunoprecipitation showed that the protein is synthesized as a precursor of relative molecular weight (Mr) 53900 ± 900 as compared to Mr 45500 ± 1000 of the mature enzyme. Translatable activity of mRNA for EPSP-synthase in glyphosate-adapted cultures is tenfold higher than in nonadapted cultures.

1 Supported by the Deutsche Forschungsgemeinschaft.






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Copyright © 1987 by the American Society of Plant Biologists