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Plant Physiology 83:251-253 (1987)
© 1987 American Society of Plant Biologists

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Environmental and Stress Physiology

Membrane Rupture Is the Common Cause of Damage to Chloroplast Membranes in Leaves Injured by Freezing or Excessive Wilting 1

Dirk K. Hincha, Roswitha Höfner, Karin B. Schwab2, Ulrich Heber and Jürgen M. Schmitt

Institut für Botanik und Pharmazeutische Biologie, Universität Würzburg, Mittlerer Dallenbergweg 64, D-8700 Würzburg, Federal Republic of Germany

The effects of freezing and desiccation of spinach leaves (Spinacia oleracea L. cv Yates) on the thylakoid membranes were assessed using antibodies specific for thylakoid membrane proteins. The peripheral part of the chloroplast coupling factor ATPase (CF1) was used as a molecular marker for chemical membrane damage by chaotropic solutes. Plastocyanin, a soluble protein localized inside the closed thylakoid membrane system, was a marker for damage by mechanical membrane rupture. After freezing and wilting of leaves which resulted in damage, very little CF1 was detached from the membranes, whereas almost all plastocyanin was released from the thylakoids. It is suggested that in vivo dehydration both by freezing and desiccation results in membrane rupture rather than in the dissociation of peripheral thylakoid membrane proteins.

2 Present address: Fakultät für Biologie, Lehrstuhl Phytopathologie, Universitätsstr. 10, D-7750 Konstanz, F.R.G.

1 Supported by the Deutsche Forschungsgemeinschaft. D. K. H. gratefully acknowledges a scholarship from the Studienstiftung des deutschen Volkes.




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[Abstract] [Full Text] [PDF]


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Copyright © 1987 by the American Society of Plant Biologists