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Development and Growth Regulation

Cytokinin Biochemistry in Relation to Leaf Senescence ¹

II. The Metabolism of 6-Benzylaminopurine in Soybean Leaves and the Inhibition of Its Conjugation

Research School of Biological Sciences, Australian National University, Canberra, A.C.T. 2601, Australia, Botany Department, University of Michigan, Ann Arbor, Michigan 48109

The metabolism of [³H]6-benzylamino purine was studied in presenescent and early senescent soybean (Glycine max [L.] Merr.) leaves. In both types of leaves, the metabolism was essentially the same. The principal metabolite was identified as -(6-benzylaminopurin-9-yl)alanine by mass spectral studies, which included discharge ionization-secondary ion mass spectrometry and pulsed positive ion-negative ion-chemical ionization mass spectrometry. Conversion to this alanine conjugate was found to be inhibited 2,4-dichlorophenoxyacetic acid and 5,7-dichloroindoleacetic acid.