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Metabolism and Enzymology

Phytic Acid Metabolism in Lily (Lilium longiflorum Thunb.) Pollen ¹

Department of Horticulture, University of Illinois, 1301 W. Gregory Dr., Urbana, Illinois 61801, Department of Biology, Washington University, St. Louis, Missouri 63130

The accumulation of phytic acid during development of lily (Lilium longiflorum Thunb.) pollen and its degradation during germination have been studied. A substantial amount of phytic acid accumulates in lily pollen by 5 days before anthesis, and little change occurs during subsequent maturation. Mature lily pollen contains 7 to 8 micrograms phytic acid per milligram pollen. Considerable degradation of phytic acid occurs by 15 minutes of incubation in glucose culture medium, and very little is left by 3 hours. No partially phosphorylated myo-inositol accumulates during germination. The breakdown of phytic acid proceeds at a constant rate during this time period. The rate is calculated to be 0.037 microgram phytic acid/milligram pollen/minute. Two phytases are detected in germinated lily pollen extract using high performance liquid chromatography with an anion exchange column (diethylaminoethyl-5PW). The results suggest that one of the phytases is already present in mature ungerminated lily pollen and the other one is newly synthesized during germination from a long-lived, pre-existing mRNA.

¹ Supported by National Science Foundation Grants 79-22686 to D. B. D. and DCB 83161319 to T.-H. D. H. and Project 65-341 of the Agricultural Experiment Station, College of Agriculture, University of Illinois at Urbana-Champaign.

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