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Metabolism and Enzymology

Evidence for Inorganic Carbon Transport by Intact Chloroplasts of Chlamydomonas reinhardtii¹

Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824, Department of Biology, Indiana University, Bloomington, Indiana 47401

Isolated intact chloroplasts from wall-less mutants of Chlamydomonas reinhardtii accumulate inorganic carbon (C_i) from the medium provided the cells had been adapted to low CO₂ photoautotrophic growth conditions. Chloroplasts from cultures grown on high (5%) CO₂ or photoheterotrophically with acetate did not accumulate inorganic carbon. Chloroplast C_i accumulation from low CO₂ grown cells was light dependent and was inhibited by uncouplers and inhibitors of electron transport. In a model for C_i accumulation by Chlamydomonas, it is proposed that CO₂ diffuses into the cell and C_i accumulation occurs in the chloroplast.

¹ Supported by National Science Foundation grant PCM 8005917 and the McKnight Foundation to N. E. T. and by National Science Foundation grant PCM 8318174 and Nippon Zeon Co. to R. K. T. Published as article 12110 of the Michigan Agricultural Experiment Station.

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