This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (34) Citing Articles via Google Scholar Google Scholar Articles by Matthys-Rochon, E. Articles by Dumas, C. Search for Related Content PubMed PubMed Citation Articles by Matthys-Rochon, E. Articles by Dumas, C. Agricola Articles by Matthys-Rochon, E. Articles by Dumas, C.

Development and Growth Regulation

Male Germ Unit Isolation from Three Tricellular Pollen Species: Brassica oleracea, Zea mays, and Triticum aestivum¹

Reconnaissance Cellulaire et Amélioration des Plantes, Université Claude Bernard, Lyon I, 43 Boulevard du 11 November 1918, 69 622 Villeurbanne Cedex, France

A technical procedure is described to follow the in vitro release of the `male germ unit' and the sperm cells from three tricellular pollen species (Brassica, Zea, and Triticum). The condition of the sperm cell was controlled using light microscopy. In addition, for the first time, the sperm cells viability has been checked by the fluorochromatic reaction test. These preliminary results indicate that this procedure appears to be a prerequisite for the successful preparation of purified viable sperm cells.

This article has been cited by other articles:

				S. Han, L. Green, and D. J. Schnell The Signal Peptide Peptidase Is Required for Pollen Function in Arabidopsis Plant Physiology, March 1, 2009; 149(3): 1289 - 1301. [Abstract] [Full Text] [PDF]

				R. Goldberg Plants: novel developmental processes Science, June 10, 1988; 240(4858): 1460 - 1467. [Abstract] [PDF]