Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 83:479-482 (1987)
© 1987 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (20)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ganson, R. J.
Right arrow Articles by Jensen, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ganson, R. J.
Right arrow Articles by Jensen, R. A.
Agricola
Right arrow Articles by Ganson, R. J.
Right arrow Articles by Jensen, R. A.
Development and Growth Regulation

Response of Cytosolic-Isozyme and Plastid-Isozyme Levels of 3-Deoxy-D-arabino-Heptulosonate 7-Phosphate Synthase to Physiological State of Nicotiana silvestris in Suspension Culture 1

Robert J. Ganson and Roy A. Jensen2

Center for Somatic-Cell Genetics and Biochemistry, State University of New York at Binghamton, Binghamton, New York 13901, Department of Biological Sciences, State University of New York at Binghamton, Binghamton, New York 13901

Two isozymes of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase are partitioned into plastid (DS-Mn) and cytosolic (DS-Co) compartments of at least several higher plants (RA Jensen 1986 Rec Adv Phytochem 20: 257-258). Differential variation of isozyme levels and in the timing of their expression was observed during growth of Nicotiana silvestris in suspension culture. The ratio of DS-Co to DS-Mn varied about fivefold in comparison of the different physiological stages of growth. Cultures maintained in exponential phase for >10 generations (EE cells) possessed balanced-growth properties and did not exhibit the considerable variation of isozyme levels found during the initial 2 to 3 generations of exponential growth (E cells) that followed subculture of stationary-phase cultures. The plastid isozyme level declined substantially in stationary phase, responded immediately to subculture, and reached a peak in early exponential growth similar to the steady-state level of DS-Mn in EE cells. In contrast, the cytosolic isozyme level peaked in late exponential growth. A recent history of stationary-phase physiology appeared to foster elevated synthesis of DS-Co since the steady-state level of DS-Co in EE cells was much lower than in E cells.

2 Present address: Department of Microbiology and Cell Science, 1059 McCarty Hall, University of Florida, Gainesville, FL 32611.

1 Supported by grant DE-AC02-78ER04967 from the Department of Energy.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1987 by the American Society of Plant Biologists