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Plant Physiology 83:640-644 (1987)
© 1987 American Society of Plant Biologists

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Metabolism and Enzymology

Phosphatidylinositol Cycle Metabolites in Samanea saman Pulvini 1

M. J. Morse, Richard C. Crain and Ruth L. Satter

Department of Molecular and Cell Biology, U-42, The University of Connecticut, Storrs, Connecticut 06268

The major metabolites of the phosphatidylinositol cycle from extracts of [32PO4]- and [3H]-inositol-labeled Samanea saman pulvini were separated. The membrane localized phosphoinositides were separated by thin layer chromatography, identified by comparison with purified lipid standards, and quantitated based on incorporation of radiolabel. The ratio of radioactivity in phosphatidylinositol:phosphatidylinositol 4-phosphate:phosphatidylinositol 4,5-bisphosphate is about 32:8:1. The aqueous inositol phosphates were separated by anion exchange chromatography using conventional liquid chromatography and by high performance liquid chromatography (HPLC) and were identified by comparison with standards. Analysis by HPLC reveals that 32P-labeled pulvini have inositol 1-phosphate, inositol 1,4-bisphosphate, and inositol 1,4,5-trisphosphate that co-migrate with red blood cell inositol phosphates, but 3H-inositol-labeled pulvini appear to have a variant profile.

1 Supported by National Science Foundation PCM 83-04613 to R. L. S., National Science Foundation DMB86-07857 to R. C. C. and R. L. S., and a University of Connecticut Research Foundation grant to R. C. C. and R. L. S.






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Copyright © 1987 by the American Society of Plant Biologists