Plant Physiol. Illumina
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Plant Physiology 83:982-987 (1987)
© 1987 American Society of Plant Biologists

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Metabolism and Enzymology

Partial Purification of Digitonin-Solubilized beta-Glucan Synthase from Red Beet Root 1

Laura L. Eiberger and Bruce P. Wasserman

Department of Food Science, New Jersey Agricultural Experiment Station, Cook College, Rutgers University, New Brunswick, New Jersey 08903

An enriched glucan synthase fraction was obtained from red beet root microsomes by sequential extraction with the detergents 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate and digitonin. The digitonin suspension was centrifuged on a glycerol gradient, where a glucan synthase peak with a specific activity of 30- to 40-fold over microsomes was recovered. Most protein contaminants were found in the gradient pellet. The glucan synthase-containing fraction was largely free of plasma membrane and tonoplast-derived ATPase activity and was enriched with a protein subunit of 68 kilodaltons.

1 Supported in part by Grant DMB 85-02523 from the National Science Foundation, a Biomedical Research Support Grant and the New Jersey Agricultural Experiment Station with State and Hatch Act Funds. New Jersey Agricultural Experiment Station, Publication No. D-10538-1-86.






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Copyright © 1987 by the American Society of Plant Biologists