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Plant Physiology 84:197-200 (1987)
© 1987 American Society of Plant Biologists

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Development and Growth Regulation

Purification of a Trypsin Inhibitor Secreted by Embryogenic Carrot Cells

Irene Carlberg, Lisbeth Jonsson, Annika Bergenstråhle and Kenneth Söderhäll1

Department of Physiological Botany, University of Uppsala, Box 540, S-751 21 Uppsala, Sweden

A protease inhibitor with a molecular weight of about 12,800 was purified to electrophoretic homogeneity from Daucus carota cells. The protease inhibitor was heat stable and inhibited trypsin but had no activity toward chymotrypsin or subtilisin. Nonembryogenic as well as embryogenic strains contained the inhibitor in similar amounts, but in the embryogenic strains the trypsin inhibitor was released from the cells and as a result accumulated in high concentrations in the culture medium, whereas no release of the trypsin inhibitor was found during cultivation of the nonembryogenic strains. Very low amounts of acid phosphatase or {alpha}-mannosidase activity were found in the culture filtrate of both embryogenic and nonembryogenic strains, which suggest that the release of the inhibitor from embryogenic strains was not due to cell lysis.

1 Supported by the Swedish Natural Science Research Council.




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S. C. de Vries, H. Booij, R. Janssens, R. Vogels, L. Saris, F. LoSchiavo, M. Terzi, and A. van Kammen
Carrot somatic embryogenesis depends on the phytohormone-controlled presence of correctly glycosylated extracellular proteins
Genes & Dev., April 1, 1988; 2(4): 462 - 476.
[Abstract] [PDF]


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Copyright © 1987 by the American Society of Plant Biologists