This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (55) Citing Articles via Google Scholar Google Scholar Articles by Cohen, E. Articles by Kende, H. Search for Related Content PubMed PubMed Citation Articles by Cohen, E. Articles by Kende, H. Agricola Articles by Cohen, E. Articles by Kende, H.

Development and Growth Regulation

In Vivo 1-Aminocyclopropane-1-Carboxylate Synthase Activity in Internodes of Deepwater Rice ¹

Enhancement by Submergence and Low Oxygen Levels

MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824

Inasmuch as the activity of 1-aminocyclopropane-1-carboxylate (ACC) synthase cannot be measured in homogenates of deepwater rice internodes (Oryza sativa L.), we have employed an in vivo assay to determine the activity of this enzyme. This assay is based on the accumulation of ACC in tissue kept under N₂. Submergence of whole plants or stem sections containing the uppermost, developing internode enhances the in vivo activity of ACC synthase in the stem. This stimulation of in vivo ACC-synthase activity is especially pronounced in the region of the internode containing the intercalary meristem and the elongation zone above it. Enhancement of in vivo ACC-synthase activity is evident after 2 hours of submergence and shows a peak after 4 hours. Reduced levels of atmospheric O₂, which promote ethylene synthesis and growth in internodes of deepwater rice, also enhance the in vivo activity of ACC synthase. Our results are consistent with the hypothesis that induction of ACC-synthase activity at low partial O₂ pressures is among the first biochemical events leading to internodal growth in deepwater rice.

¹ Supported by the National Science Foundation through Grant No. DCB 8416492 and by the United States Department of Energy under Contract No. DE-AC02-76ER01338.

This article has been cited by other articles:

				S.-I. Oh, J. Park, S. Yoon, Y. Kim, S. Park, M. Ryu, M. J. Nam, S. H. Ok, J.-K. Kim, J.-S. Shin, et al. The Arabidopsis Calcium Sensor Calcineurin B-Like 3 Inhibits the 5'-Methylthioadenosine Nucleosidase in a Calcium-Dependent Manner Plant Physiology, December 1, 2008; 148(4): 1883 - 1896. [Abstract] [Full Text] [PDF]

				M. B. Jackson Ethylene-promoted Elongation: an Adaptation to Submergence Stress Ann. Bot., January 1, 2008; 101(2): 229 - 248. [Abstract] [Full Text] [PDF]

				G. Rzewuski, K. A. Cornell, L. Rooney, K. Burstenbinder, M. Wirtz, R. Hell, and M. Sauter OsMTN encodes a 5'-methylthioadenosine nucleosidase that is up-regulated during submergence-induced ethylene synthesis in rice (Oryza sativa L.) J. Exp. Bot., April 1, 2007; 58(6): 1505 - 1514. [Abstract] [Full Text] [PDF]

				H. Saika, M. Okamoto, K. Miyoshi, T. Kushiro, S. Shinoda, Y. Jikumaru, M. Fujimoto, T. Arikawa, H. Takahashi, M. Ando, et al. Ethylene Promotes Submergence-Induced Expression of OsABA8ox1, a Gene that Encodes ABA 8'-Hydroxylase in Rice Plant Cell Physiol., February 1, 2007; 48(2): 287 - 298. [Abstract] [Full Text] [PDF]

				I. Rieu, S. M. Cristescu, F. J. M. Harren, W. Huibers, L. A. C. J. Voesenek, C. Mariani, and W. H. Vriezen RP-ACS1, a flooding-induced 1-aminocyclopropane-1-carboxylate synthase gene of Rumex palustris, is involved in rhythmic ethylene production J. Exp. Bot., March 1, 2005; 56(413): 841 - 849. [Abstract] [Full Text] [PDF]

				M. Sauter, K. A. Cornell, S. Beszteri, and G. Rzewuski Functional Analysis of Methylthioribose Kinase Genes in Plants Plant Physiology, December 1, 2004; 136(4): 4061 - 4071. [Abstract] [Full Text] [PDF]

				W. H. VRIEZEN, Z. ZHOU, and D. VAN DER STRAETEN Regulation of Submergence-induced Enhanced Shoot Elongation in Oryza sativa L. Ann. Bot., January 2, 2003; 91(2): 263 - 270. [Abstract] [Full Text] [PDF]

				Z. Zhou, J. de Almeida Engler, D. Rouan, F. Michiels, M. Van Montagu, and D. Van Der Straeten Tissue Localization of a Submergence-Induced 1-Aminocyclopropane-1-Carboxylic Acid Synthase in Rice Plant Physiology, May 1, 2002; 129(1): 72 - 84. [Abstract] [Full Text] [PDF]

				D. Van Der Straeten, Z. Zhou, E. Prinsen, H. A. Van Onckelen, and M. C. Van Montagu A Comparative Molecular-Physiological Study of Submergence Response in Lowland and Deepwater Rice Plant Physiology, February 1, 2001; 125(2): 955 - 968. [Abstract] [Full Text]

				W. H. Vriezen, R. Hulzink, C. Mariani, and L. A.C.J. Voesenek 1-Aminocyclopropane-1-Carboxylate Oxidase Activity Limits Ethylene Biosynthesis in Rumex palustris during Submergence Plant Physiology, September 1, 1999; 121(1): 189 - 196. [Abstract] [Full Text]

				R. Lorbiecke and M. Sauter Adventitious Root Growth and Cell-Cycle Induction in Deepwater Rice Plant Physiology, January 1, 1999; 119(1): 21 - 30. [Abstract] [Full Text]