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Plant Physiology 84:399-403 (1987)
© 1987 American Society of Plant Biologists

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Metabolism and Enzymology

Purification and Properties of Acid Phosphatase-1 from a Nematode Resistant Tomato Cultivar

Elizabeth M. Paul1 and Valerie M. Williamson2

ARCO Plant Cell Research Institute, Dublin, California 94568

In tomato the acid phosphatase-1 isozyme (Apase-1) is inherited as a single locus linked to the nematode resistance gene (Mi). The Apase-11 electrophoretic variant has been purified from a tomato cell suspension culture using ion exchange and concanavalin A sepharose affinity chromatography. A cellulose acetate electrophoresis method was used to distinguish Apase-11 rapidly from other Apase isozymes in tomato. The subunit molecular weight of the purified enzyme was estimated to be 31,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The native size of the enzyme, which is reported to be a dimer, was determined to be approximately 51,000 by high performance liquid chromatography gel filtration. Apase-11 has a lower pH optimum and a distinct substrate specificity as compared to Apases extracted from tomato fruit or from other plant species. The amino acid composition of Apase-11 is similar to that of a potato Apase.

1 Present address: Botany Department, Leicester University, Leicester, LE 17 RH, England.

2 Present address: Division of Nematology, University of California, Davis, CA 95616.




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J.L. Jakobek and P.B. Lindgren
Expression of a bean acid phosphatase cDNA is correlated with disease resistance
J. Exp. Bot., February 1, 2002; 53(367): 387 - 389.
[Abstract] [Full Text] [PDF]


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Copyright © 1987 by the American Society of Plant Biologists