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Plant Physiology 84:1051-1054 (1987)
© 1987 American Society of Plant Biologists

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Environmental and Stress Physiology

Effect of Paclobutrazol on Water Stress-Induced Abscisic Acid in Apple Seedling Leaves

Shiow Y. Wang, Tung Sun, Zuo L. Ji1 and Miklos Faust

Fruit Laboratory, Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705, Food Safety Inspection Service, United States Department of Agriculture, P. O. Box 6085, Athens, Georgia 30604

Abscisic acid (ABA) was quantitated by enzyme-linked immunosorbent assay (ELISA) in water-stressed leaves from control apple seedlings, and also from apple seedlings treated for 28 days with paclobutrazol ([2RS, 3RS]-1-[4-chlorophenyl]-4,4-dimethyl-2-[1,2,4-triazol-1-yl] pentan-3-ol). The ELISA quantitative estimates were also validated by gas chromatography-electron capture detector and lettuce seed germination inhibition bioassay. Paclobutrazol treatment reduced endogenous ABA levels by about one-third, and prevented the marked accumulation of water-stress-induced ABA that occurred in untreated seedlings. The presence of ABA in the apple leaf extracts was confirmed by gas chromatography-mass spectrometry.


1 On leave from the Department of Horticulture, South China Agricultural University, Guangzhou, People's Republic of China.




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Copyright © 1987 by the American Society of Plant Biologists