Plant Physiology 84:1270-1275 (1987)
© 1987 American Society of Plant Biologists
Environmental and Stress Physiology
Salt Stress Increases the Level of Translatable mRNA for Phosphoenolpyruvate Carboxylase in Mesembryanthemum crystallinum1
James A. Ostrem,
Steve W. Olson,
Jürgen M. Schmitt2 and
Hans J. Bohnert
Department of Biochemistry, University of Arizona, Biosciences West, Tucson, Arizona 85721
Mesembryanthemum crystallinum responds to salt stress by switching from C3 photosynthesis to Crassulacean acid metabolism (CAM). During this transition the activity of phosphoenolpyruvate carboxylase (PEPCase) increases in soluble protein extracts from leaf tissue. We monitored CAM induction in plants irrigated with 0.5 molar NaCl for 5 days during the fourth, fifth, and sixth week after germination. Our results indicate that the age of the plant influenced the response to salt stress. There was no increase in PEPCase protein or PEPCase enzyme activity when plants were irrigated with 0.5 molar NaCl during the fourth and fifth week after germination. However, PEPCase activity increased within 2 to 3 days when plants were salt stressed during the sixth week after germination. Immunoblot analysis with anti-PEPCase antibodies showed that PEPCase synthesis was induced in both expanded leaves and in newly developing axillary shoot tissue. The increase in PEPCase protein was paralleled by an increase in PEPCase mRNA as assayed by immunoprecipitation of PEPCase from the in vitro translation products of RNA from salt-stressed plants. These results demonstrate that salinity increased the level of PEPCase in leaf and shoot tissue via a stress-induced increase in the steady-state level of translatable mRNA for this enzyme.
2 Present address: Botanisches Institut der Universität Würzburg, Mittlerer Dallenbergweg, D 8700 Würzburg, Federal Republic of Germany.
1 Supported by grants from the United States Department of Agriculture (85-CRCR-1-1677) to H. J. B., Deutsche Forschungsgemeinschaft and Deutscher Akademischer Austauschdienst to J. M. S. Travel was supported by NATO Collaborative Research grant RG230/84 to J. M. S. and H. J. B.
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