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Microbe Plant Interactions

Induction of Defense Responses in Cultured Parsley Cells by Plant Cell Wall Fragments ¹

Complex Carbohydrate Research Center, University of Georgia, P. O. Box 5677, Athens, Georgia 30613, Max-Planck-Institut für Züchtungsforschung, Abteilung Biochemie, D-5000 Köln 30, Federal Republic of Germany

Cell suspension cultures of parsley (Petroselinum crispum) accumulated coumarin phytoalexins and exhibited increased -1,3-glucanase activity when treated with either a purified -1,4-D-endopolygalacturonic acid lyase from Erwinia carotovora or oligogalacturonides solubilized from parsley cell walls by endopolygalacturonic acid lyase. Coumarin accumulation induced by the plant cell wall elicitor was preceded by increases in the activities of phenylalanine ammonia lyase (PAL), 4-coumarate:CoA ligase (4CL) and S-adenosyl-L-methionine:xanthotoxol O-methyltransferase (XMT). The time courses for the changes in these three enzyme activities were similar to those observed in cell cultures treated with a fungal glucan elicitor. The plant cell wall elicitor was found to act synergistically with the fungal glucan elicitor in the induction of coumarin phytoalexins. As much as a 10-fold stimulation in coumarin accumulation above the calculated additive response was observed in cell cultures treated with combinations of plant and fungal elicitors. The synergistic effect was also observed for the induction of PAL, 4CL, and XMT activities. These results demonstrate that plant cell wall elicitors induce at least two distinct biochemical responses in parsley cells and further support the role of oligogalacturonides as important regulators of plant defense.

¹ Supported by the Max-Planck-Society, Fonds der Chemischen Industrie (grant to K. H.) and National Science Foundation DMB-8518488 awarded to Peter Albersheim.

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