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Plant Physiology 84:993-996 (1987)
© 1987 American Society of Plant Biologists

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Membranes and Bioenergetics

Transport Properties of the Tomato Fruit Tonoplast 1

I. Identification and Characterization of an Anion-Sensitive H+-ATPase

Nancy Oleski2, Peiman Mahdavi, Galen Peiser3 and Alan B. Bennett

Mann Laboratory, Department of Vegetable Crops, University of California, Davis, California 95616

An anion-sensitive H+-translocating ATPase was identified in membrane vesicles isolated from mature green tomato (Lycopersicon esculentum) fruit. The H+-ATPase was associated with a low density membrane population having a peak density of 1.11 grams per cubic centimeter, and its activity was inhibited by NO3–, N,N'-dicyclohexylcarbodiimide and diethylstilbestrol but not by vanadate, azide, molybdate, or oligomycin. This H+-ATPase has an unusual pH dependence indicating both a slightly acidic and a near neutral peak of activity. Chloride was found to be a potent stimulator of ATPase activity. The Km for the H+-ATPase was approximately 0.8 millimolar ATP. The characteristics of this H+-ATPase are very similar to those described for a number of plant cell tonoplast H+-ATPases suggesting that the activity identified in tomato fruit membranes is tonoplast-associated. This report demonstrates the feasibility of isolating tonoplast vesicles from acidic fruit tissues for studies of transport activities associated with fruit development and maturation.

2 Present address: Plant Biology Laboratory, The Salk Institute for Biological Studies, P. O. Box 85800, San Diego, CA 92138-9216.

3 Present address: NPI, 417 Wakara Way, Salt Lake City, UT 84108.

1 Supported by National Science Foundation grant DMB 84-04990.




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P. Lobit, M. Genard, P. Soing, and R. Habib
Modelling malic acid accumulation in fruits: relationships with organic acids, potassium, and temperature.
J. Exp. Bot., January 1, 2006; 57(6): 1471 - 1483.
[Abstract] [Full Text] [PDF]


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Copyright © 1987 by the American Society of Plant Biologists