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Plant Physiology 84:997-1000 (1987)
© 1987 American Society of Plant Biologists

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Membranes and Bioenergetics

Transport Properties of the Tomato Fruit Tonoplast 1

II. Citrate Transport

Nancy Oleski2, Peiman Mahdavi and Alan B. Bennett

Mann Laboratory, Department of Vegetable Crops, University of California, Davis, California 95616

Citrate transport across the membrane of tomato fruit tonoplast vesicles was investigated. In the tonoplast vesicles, [14C]methylamine uptake was stimulated 10-fold by MgATP and strongly inhibited by NO3–. Under identical experimental conditions, [14C]citrate uptake was inhibited by 5 millimolar free Mg2+, and this inhibition was reversed in the presence of ATP, presumably by ATP chelation of free Mg2+. No evidence was obtained in support of energy-linked ATP stimulation of citrate uptake. Citrate uptake showed saturation kinetics, and was inhibited by 4,4'-diisothiocyano-2,2'-stilbenedisulfonic acid and by other organic acids. The pH-dependence of uptake suggested that citrate3– was the transported species. Our results indicate that citrate transport across the tomato fruit tonoplast occurs by facilitated diffusion of citrate3–. The carrier shares some features in common with anion channels in that it is relatively nonspecific for organic acids and is inhibitable by 4,4'-diisothyocyano-2,2'-stilbenedisulfonic acid.

2 Present address: Plant Biology Laboratory, The Salk Institute for Biological Studies, P.O. Box 85800, San Diego, CA 92138-9216.

1 Supported by National Science Foundation grant DMB 84-04990.




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Copyright © 1987 by the American Society of Plant Biologists