Plant Physiology 85:62-65 (1987)
© 1987 American Society of Plant Biologists
Environmental and Stress Physiology
Compartmental Efflux Analysis and Removal of Extracellular Cadmium from Roots 1
Wilfried E. Rauser
Department of Botany, University of Guelph, Guelph, Ontario NIG 2W1 Canada
Profiles of 109Cd efflux from roots into three solutions were determined for young intact plants of Agrostis gigantea and maize. The solutions were (a) nutrient culture medium containing 3 micromolar Cd at room temperature, (b) ice-cold 5 millimolar CaCl2, and (c) ice-cold 5 millimolar PbCl2. Efflux profiles were clearly resolved into three easily discernible components having fast, medium, and slow exchange rates. These results were unexpected for the situation where some intracellular Cd was present both as extractable Cd-binding peptide and in electron-dense granules within the cytoplasm and the vacuoles. Adding a fourth compartment to the curve-fitting model produced a splitting of the fast exchanging component. Use of these efflux kinetics to estimate Cd fluxes through membranes was inappropriate. However, they were useful in determining optimal washing times for the removal of extracellular Cd. A 10 minute wash in ice-cold 5 millimolar CaCl2 is recommended for this purpose for Agrostis and maize roots.
1 Supported by Operating Grant A4921 from the Natural Sciences and Engineering Research Council of Canada.
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