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Development and Growth Regulation

Identification of Endogenous Gibberellins from Oilseed Rape ¹

Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta, T1K 3M4, Canada, Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, Canada

Oilseed rape (Brassica napus, canola variety `Westar') plants were grown in greenhouse conditions and shoots were harvested during the final stages of shoot elongation. Leaves and immature pods were removed and the remaining stem tissue was extracted and purified. The extract was chromatographed on sequential, step-eluted silica gel partition and reverse-phase C<sub>18</sub> HPLC columns, and gibberellin (GA)-like substances were detected using the `Tan-ginbozu' dwarf rice microdrop assay. Purified fractions showing GA-like activity were analyzed by capillary gas chromatography-mass spectrometry (GC-MS) and GC-selected ion monitoring (GC-SIM). Gibberellins A₁, A₃, and iso-A₃ were identified by full spectrum GC-MS with GA₁ being the most abundant GA in the stem tissue. Gibberellins A₁₉ and A₂₀ were identified by GC-SIM and are logical precursors of the GA₁.