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Plant Physiology 85:699-705 (1987)
© 1987 American Society of Plant Biologists

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Environmental and Stress Physiology

Alteration of Gene Expression during the Induction of Freezing Tolerance in Brassica napus Suspension Cultures 1

Anne M. Johnson-Flanagan2 and Jas Singh

Plant Research Centre, Agriculture Canada, Ottawa, Ontario K1A 0C6, Canada

Brassica napus suspension-cultured cells can be hardened to a lethal temperature for 50% of the sample of –20°C in eight days at room temperature with abscisic acid. During the induction of freezing tolerance, changes were observed in the electrophoretic pattern of [35S]methionine labeled polypeptides. In hardening cells, a 20 kilodalton polypeptide was induced on day 2 and its level increased during hardening. The induction of freezing tolerance with nonmaximal hardening regimens also resulted in increases in the 20 kilodalton polypeptide. The 20 kilodalton polypeptide was associated with a membrane fraction enriched in endoplasmic reticulum and was resolved as a single spot by two-dimensional electrophoresis. In vitro translation of mRNA indicate alteration of gene expression during abscisic acid induction of freezing tolerance. The new mRNA encodes a 20 kilodalton polypeptide associated with increased freezing tolerance induced by either abscisic acid or high sucrose. A 20 kilodalton polypeptide was also translated by mRNA isolated from cold-hardened B. napus plants.

2 Present address: Department of Plant Science, University of Alberta, Edmonton, Alberta, T6G 2P5, Canada.

1 Supported, in part, by an Agriculture Canada Visiting Scientist in Biotechnology Fellowship to A. M. J.-F. Contribution No. 1069 of the Plant Research Centre.






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Copyright © 1987 by the American Society of Plant Biologists